Biology · Glossary

What is Actin filaments, microtubules, intermediate filaments?

Definition 9.1 University Biology — Year 3 · Chapter 9 — Cytoskeleton Dynamics and Cell Motility

An actin filament is a two-stranded helical polymer of globular actin, 7nm7\,\mathrm{nm} thick, each subunit 2.7nm2.7\,\mathrm{nm} along the axis, polar: the barbed (plus) end grows faster than the pointed (minus) end, and each subunit carries an ATP that is hydrolysed after incorporation. A microtubule is a hollow tube of 25nm25\,\mathrm{nm} outer diameter built from thirteen protofilaments of αβ\alpha\beta-tubulin dimers (8nm8\,\mathrm{nm} per dimer), also polar, its plus end growing faster and its minus end usually anchored at the centrosome near the nucleus; the β\beta subunit hydrolyses its GTP after incorporation. An intermediate filament is a rope of 10nm10\,\mathrm{nm} made of coiled-coil proteins (keratins in epithelia, vimentin in mesenchyme, neurofilaments in axons, lamins under the nuclear envelope), apolar, without nucleotide, and far more stable — a tension-bearing cable rather than a dynamic track. Actin sits mostly under the plasma membrane and in protrusions; microtubules radiate from the centrosome and serve as the tracks of long-range transport and as the spindle; intermediate filaments give a cell and its nucleus their mechanical resilience.

The three filaments to scale in diameter. Actin and tubulin polymers are polar and hydrolyse a nucleotide after assembly, which is what makes them dynamic; the intermediate filament is an apolar rope built for endurance.
The three filaments to scale in diameter. Actin and tubulin polymers are polar and hydrolyse a nucleotide after assembly, which is what makes them dynamic; the intermediate filament is an apolar rope built for endurance.
Left: actin (green) in a migrating fibroblast — stress fibres across the body, a dense meshwork at the broad leading edge on the right. Right: microtubules (orange) radiating from the centrosome beside the nucleus (blue) to the cell margin. Left: actin (green) in a migrating fibroblast — stress fibres across the body, a dense meshwork at the broad leading edge on the right. Right: microtubules (orange) radiating from the centrosome beside the nucleus (blue) to the cell margin.
Left: actin (green) in a migrating fibroblast — stress fibres across the body, a dense meshwork at the broad leading edge on the right. Right: microtubules (orange) radiating from the centrosome beside the nucleus (blue) to the cell margin.
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