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Chemistry · Glossary

What is Retention?

Also known as: retention time · hold-up time · retention factor k

Definition 31.2 University Chemistry — Year 2 · Chapter 31 — Chromatography

The retention time tRt_R of a compound is the time from injection to the maximum of its peak; the hold-up time tMt_M is the retention time of an unretained compound, which never enters the stationary phase. The retention factor kk of a compound on a column is

k=tR−tMtM.k = \frac{t_R - t_M}{t_M}.

It is not the RfR_f of a thin-layer plate, which measures a distance travelled, though both describe the same partition.

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